MGC803-CAS9 cell line

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  • MGC803-CAS9 cell line MGC803-CAS9-产品图
MGC803-CAS9 cell line MGC803-CAS9-产品图
MGC803-CAS9 Copy Product Info
* Hela-contaminated cells, not for sales
Catalog#： YC-C081-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
  • MGC803-CAS9 cell line MGC803-CAS9-产品图
MGC803-CAS9 Copy Product Info
* Hela-contaminated cells, not for sales
Catalog#： YC-C081-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like,adherent Culture method 90%DMEM+10%FBS
Passage ratio 1:3~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like,adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's MGC803 cell line was constructed on the basis of wild-type MGC803 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in MGC803 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MGC803		 STR Info (Cell bank)
Cell Line:MGC-803
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
D5S818 101112.0101112.0
D13S317 713.3713.3
D7S820 11121112
D16S539 911911
VWA 1618161718.0
TH01 7979
AMEL XXXX
TPOX 12121212
CSF1PO 91012.091012.0
D12S391 2026
FGA 1821
D2S1338 1724
D21S11 2728
D18S51 1313
D8S1179 1214
D3S1358 1518
D6S1043 1819
PENTAE 71016.017.0
D19S433 1313
PENTAD 815
D1S1656 121516.0
* Hela-contaminated cells, not for sales

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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